Reconstitution of microtubule nucleation in vitro reveals novel roles for Mzt1
Sawin lab paper featured in Current Biology.
Authors
Leong SL, Lynch EM, Zou J, Tay YD, Borek WE, Tuijtel MW, Rappsilber J, Sawin KE.
Summary
Leong et al. reconstitute microtubule nucleation in vitro with a minimal set of purified fission yeast proteins--Mto1/2[bonsai] complex, heterotetrameric g-TuSC and Mzt1--that assemble into a ring-like "MGM" holocomplex. The small conserved protein Mzt1 is implicated in stabilization of Alp6, fission yeast ortholog of mammalian g-TuSC protein GCP3.