Centromere DNA destabilizes H3 nucleosomes to promote CENP-A deposition during the cell cycle
Allshire lab paper featured in Current Biology.
Authors
Shukla, M.,Tong, P.,White, S.A., Singh, P.P., Reid, A.M.,Catania,S., Pidoux, A.L., Allshire, R.C.
Current Biology (2018) 28: 3924–3936
Summary
Histone H3 levels increase at centromeres during S phase, new CENP-A replaces H3 during G2.
Centromere DNA drives H3 cell cycle dynamics independent of centromere proteins and the eviction of H3 is coincident with recruitment of elongating RNAPII. We propose a model in which RNAPII-coupled chromatin remodeling promotes replacement of H3 with CENP-A nucleosome