Transcriptome-wide RNA processing kinetics revealed using extremely short 4tU labeling
Beggs lab paper featured in Genome Biology.
Authors
Barrass, J.D., Reid, J.E.A., Huang, Y., Hector, R.D., Sanguinetti, G., Beggs, J.D. and Granneman, S.
Summary
Barrass et al describe an optimised metabolic labelling method that allows the measurement of nascent RNA metabolism in yeast with unprecedented kinetic resolution. Features were identified in RNA that affect pre-mRNA splicing kinetics and turnover of non-coding RNAs.