Loss of the yeast SR protein Npl3 alters gene expression due to transcription readthrough
Tollervey lab paper featured in PLoS Genetics.
Authors
Holmes, R.K., Tuck, A.C., Zhu, C., Dunn-Davies, H.R., Kudla, G., Clauder-Munster, S., Granneman, S., Steinmetz, L.M., Guthrie, C. and Tollervey, D.
Summary
This paper reports that loss of the of major pre-mRNA binding protein Npl3 resulted in transcriptional termination defects at diverse RNAs in yeast , with readthrough observed on large subsets of both mRNAs and ncRNAs. These termination cause widespread changes in gene expression, both through inappropriate termination and through transcriptional interference at neighbouring genes.