Architecture of the RNA polymerase II-TFIIF complex revealed by cross-linking and mass spectrometry
Rappsilber lab paper featured in EMBO Journal.
Authors
Chen ZA, Jawhari A, Fischer L, Buchen C, Tahir S, Kamenski T, Rasmussen M, Lariviere L, Bukowski-Wills JC, Nilges M, Cramer P, Rappsilber J.
From cores to peripheries
Cross-linking and mass spectrometry provide the distance constrains between yeast transcription factor IIF and RNA polymerase II that allow docking a model of a transcription factor fragment onto the crystal structure of Pol II and extend the core complex of the transcription machinery towards its peripheries.