Nikon Ti2 Spinning Disk Confocal

Tutorial for Nikon Ti2 Spinning Disk Confocal startup and shutdown.

Start Up

1. Remove cover, do not put the cover on the floor!

Photo of Nikon Spinning Disk Confocal equipment — Camera to LHS of microscope

2. Turn on camera

3. Turn on spinning disk, blue box to LHS of microscope and key

4. Turn on the main power and then the microscope

5. Turn on laser box.

Then turn on the key, bottom shelf below spinning disk and microscope power boxes

6. Turn on the piezo, on the top shelf

7. Check the camera has stopped showing a flashing light.

Then turn on the digital trigger box on top shelf, this will also turn on the computer

8. Turn on White light (CoolLED)

Shutdown

1. Close software and shutdown computer

2. Remove your sample and if you have used an oil objective gently clean the oil from the objective using lens tissue and ethanol, do not use the 70% ethanol or tissue paper these are provided for cleaning work surfaces

3. Turn off microscope, wait for lights on the front to stop flashing before turning off the microscope main power box

4. Turn off White light, Piezo, Laser box (key then power), Spinning Disk (key and then blue box), Camera and digital trigger box

5. Replace microscope cover

This article was published on 13 Feb, 2023