Haematoxylin and Eosin

Haematoxylin and Eosin protocols.

Paraffin Sections

• Rehydrate
  • 2 x xylene or histoclear

  • 2 x absolute alcohol

  • 2 x 70% alcohol

• Ehrlichs haematoxylin (5 mins)

• Rinse in tap water

• Differentiate in acid alcohol (15 sec)

• Blue in tap water (5 mins)

• Aqueous eosin (30ml eosin, 300ml water) for 15 mins

• Rinse in tap water

• Dehydrate

  • 2 x 70% alcohol

  • 2 x absolute alcohol

  • 2 x xylene or histoclear

• Mount in DPX

Frozen Sections

• Rehydrate slides in tap water (1 min)

• Full strength Ehrlichs haematoxylin (40 secs)

• Gently rinse in tap water

• Dip 3 times in acid alcohol

• Gently rinse in tap water (5 mins)

• Aqueous eosin (30ml eosin, 300ml water) for 15 mins

• Rinse gently in tap water (5 mins)

• Dehydrate

  • 2 x 70% alcohol

  • 2 x absolute alcohol

  • 2 x xylene or histoclear

• Mount in DPX